Archive of SID Telomerase Activity in Chicken Embryo Fibroblast Cell Cultures Infected with Marek's Disease Virus
Year: 2010
Type: article
Abstract: Background:Telomerase is a ribonucleoprotein, which adds telomeric repeats onto the 3’end of existing telomers at the end of chromosomes ineukaryotes. One hypothesis states that telomere length may function as a mitoticclock, therefore expression of telomerase activity in cancer cells may be a necessary and essential step for tumor development and progression.Methods:The detectability of telomerase activity in chicken embryofibroblast (CEF) cells infected with different passages of Marek's disease virus(MDV) was tested with the TRAPEZE® telomerase detection kit at passages14 (P14), P80/1 and P120 for the Woodland strain, and passage 9 (P9) for theMPF57 strain. Results:The results showed increased telomerase activity in MDV Woodlands strain at P14 and MPF57 strain at P9. Conclusion:Our results suggest that MDV-transformed cells at low passage are a suitable system for the study of telomerases in tumor developmentand for testing telomerase-inhibiting drugs.
Authors: Mohammad Motamedifar, Gregory A. Tannock
Institutions: Shiraz University of Medical Sciences, RMIT University
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W1771689159