Work

Competition Between Splicing and Polyadenylation Reactions Determines which Adenovirus Region E3 mRNAs Are Synthesized

Year: 1988

Type: article

Abstract: Complex transcription units encode multiple mRNAs which arise by alternative processing of a common pre-mRNA precursor. It is not known how the pre-mRNA processing pathways are determined or controlled. We are investigating this problem by using the E3 complex transcription unit of adenovirus as a model. Our approach is to construct virus mutants with lesions in E3 and then determine how the mutation affects the accumulation of E3 mRNAs in vivo. We report results which indicate that competition between splicing reactions and polyadenylation reactions occurs in vivo and that this plays an important role in alternative pre-mRNA processing.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.8.8.3291

Authors: H A Brady, W S Wold

Institution: Saint Louis University

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Science Foundation

Award: Picosecond Spectroscopy of Carbon-Heteroatom Bond- Dissociation Reactions