Formate Dehydrogenase of Clostridium thermoaceticum : Incorporation of Selenium-75, and the Effects of Selenite, Molybdate, and Tungstate on the Enzyme
Year: 1973
Type: article
Abstract: The formation of the nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase in Clostridium thermoaceticum is stimulated by the presence of molybdate and selenite in the growth medium. The highest formate dehydrogenase activity was obtained with 2.5 x 10(-4) M Na(2)MoO(4) and 5 x 10(-5) Na(2)SeO(3). Tungstate but not vanadate could replace molybdate and stimulate the formation of formate dehydrogenase. Tungstate stimulated activity more than molybdate, and in combination with molybdate the stimulation of formation of formate dehydrogenase was additive. Formate dehydrogenase was isolated from cells grown in the presence of Na(2) (75)SeO(2), and a correlation was observed between bound (75)Se and enzyme activity.
Source: Journal of Bacteriology
Authors: Jan R. Andreesen, Lars G. Ljungdahl
Institution: University of Georgia
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1786577940