Localized mutagenesis with bacteriophage Mu: method for increasing the frequency of specific bacterial mutants
Year: 1977
Type: article
Abstract: A method is described for markedly enriching a bacterial population for cells containing any given Mu insertion mutation. The method involves the transfer of a small piece of deoxyribonucleic acid from a Mu-infected Hfr donor donor strain to a suitable F- strain and a subsequent selection of those recombinant organisms that have received a Mu prophage from the donor. The method is particularly usefule for isolating mutants whose selection requires "brute-force" assay, since only a few hundred colonies have to be screened.
Source: Journal of Bacteriology
Authors: H Tabor, E W Hafner, C W Tabor
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W1788968033