Year: 1979
Type: article
Abstract: 1. A method is described that allows for measurement of protein synthesis in liver and intestine in the rat. By injecting a massive amount of [14C]leucine (100 mumol/100 g body wt.) an attempt has been made to over come problems of precursor specific radioactivity and problems arising from the breakdown of labelled protein that are encountered when tracer amounts of amino acids are used. 2. Starvation for 2 days resulted in decline in the rate of total liver protein synthesis from 87%/day to 62%/day. 3. In jejunal mucosa the rate of protein synthesis was 136%/day. This declined to 105%/day after 2 days of starvation.
Source: Biochemical Journal
DOI: 10.1042/bj1780373
Authors: M A McNurlan, A M Tomkins, P J Garlick
Institution: London School of Hygiene & Tropical Medicine
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Funders: Wellcome Trust, Royal Society
OpenAlex ID: W1806560945