Work

Human Polymorphonuclear Leukocytes Produce IL-12, TNF-α, and the Chemokines Macrophage-Inflammatory Protein-1α and -1β in Response to Toxoplasma gondii Antigens

Year: 1999

Type: article

Abstract: The induction of a type 1 inflammatory cytokine response is a key event in the initiation of immunity to Toxoplasma gondii. Because polymorphonuclear leukocytes rapidly respond to infection by exiting the peripheral blood and accumulating at a site of infection, we sought to determine whether these cells produce cytokines in response to T. gondii. When human peripheral blood neutrophils were stimulated with parasite Ag, they produced both IL-12 (p70) and TNF-alpha. Similarly, up-regulated expression of macrophage-inflammatory protein-1 alpha (MIP-1 alpha) and MIP-1 beta gene transcripts was induced. Kinetic analysis of IL-12 and TNF-alpha production revealed distinct patterns following stimulation by T. gondii or LPS. Exogenous TNF-alpha alone also provided a potent stimulus of MIP-1 alpha and MIP-1 beta expression, and when neutralizing anti-TNF-alpha antiserum was included in cultures of parasite-stimulated cells, expression of these CC-family chemokines was partially blocked. These results establish that T. gondii possesses the ability of driving neutrophil proinflammatory cytokine production, and they suggest that parasite-induced MIP-1 alpha and MIP-1 beta partly results from autocrine stimulation through TNF-alpha.

Source: The Journal of Immunology

DOI: 10.4049/jimmunol.162.12.7369

Authors: Susan K Bliss, Anthony J Marshall, Yin Zhang, Eric Y Denkers

Institutions: Cornell University, New York State College of Veterinary Medicine

Language: English

Topic: Toxoplasma gondii Research Studies

Subfield: Parasitology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: U.S. Department of Agriculture, National Institutes of Health, National Institute of Allergy and Infectious Diseases