Retrovirus-Mediated Transfer of an Adenovirus Gene Encoding an Integral Membrane Protein Is Sufficient To Down Regulate the Receptor for Epidermal Growth Factor
Year: 1990
Type: article
Abstract: We have used retrovirus-mediated gene transfer to introduce sequences encoding a 10,400-molecular-weight (10.4K) adenovirus protein previously shown to down regulate the receptor for epidermal growth factor (EGF) into two murine cell lines that possess human EGF receptors (EGF-Rs). Assays for receptor expression showed that acute infection resulted in rapid, constitutive down regulation of the EGF-R via a pathway that appears to be endosome mediated. This represents the first demonstration that 10.4K expression in the absence of other virus-encoded proteins is sufficient to elicit this response. The usefulness of this approach for the study of 10.4K-mediated signal transduction in cells with a nontransformed phenotype is discussed.
Source: Molecular and Cellular Biology
Authors: B L Hoffman, A Ullrich, W S Wold, C R Carlin
Institution: Saint Louis University
Language: English
Topic: HER2/EGFR in Cancer Research
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W1829732864