Alzheimer's in 3D culture: Challenges and perspectives
Year: 2015
Type: review
Abstract: Alzheimer's disease (AD) is the most common cause of dementia, and there is currently no cure. The "β-amyloid cascade hypothesis" of AD is the basis of current understanding of AD pathogenesis and drug discovery. However, no AD models have fully validated this hypothesis. We recently developed a human stem cell culture model of AD by cultivating genetically modified human neural stem cells in a three-dimensional (3D) cell culture system. These cells were able to recapitulate key events of AD pathology including β-amyloid plaques and neurofibrillary tangles. In this review, we will discuss the progress and current limitations of AD mouse models and human stem cell models as well as explore the breakthroughs of 3D cell culture systems. We will also share our perspective on the potential of dish models of neurodegenerative diseases for studying pathogenic cascades and therapeutic drug discovery.
Source: BioEssays
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G1185037961, Presenilin Biology and the Mechanisms of Alzheimer's Disease, G2161184881, Identification and Characterization of Novel AD Genes, A novel human 3D neural cell culture system for the characterization of AD genes, G5099711879, G6247371286, G6502547433, Massachusetts Alzheimer's Disease Research Center, Alzheimer's BACE1 inhibition regulates neuronal contactin function, Age-related changes of cognition in health and disease, G945945448
OpenAlex ID: W1830876320