Work

Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria

Year: 1985

Type: article

Abstract: We constructed the broad-host-range plasmid pUCD800 containing the sacB gene of Bacillus subtilis for use in the positive selection and isolation of insertion sequence (IS) elements in gram-negative bacteria. Cells containing pUCD800 do not grow on medium containing 5% sucrose unless the sacB gene is inactivated. By using pUCD800, we isolated a 1.4-kilobase putative IS element from Agrobacterium tumefaciens NT1RE by selection for growth on sucrose medium. This putative IS element appears to be unique to Agrobacterium strains.

Source: Journal of Bacteriology

DOI: 10.1128/jb.164.2.918-921.1985

Authors: P Gay, D Le Coq, M Steinmetz, T Berkelman, C I Kado

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Cancer Institute

Award: TUMOR INDUCING SUBSTANCE OF AGROBACTERIUM TUMEFACIENS

OpenAlex ID: W1832001947