Formation of Spatially and Geometrically Controlled Three-Dimensional Tissues in Soft Gels by Sacrificial Micromolding
Year: 2014
Type: article
Abstract: Patterned three-dimensional (3D) cell culture models aim to more accurately represent the in vivo architecture of a tissue for the purposes of testing drugs, studying multicellular biology, or engineering functional tissues. However, patterning 3D multicellular structures within very soft hydrogels (<500 Pa) that mimic the physicochemical environment of many tissues remains a challenge for existing methods. To overcome this challenge, we use a Sacrificial Micromolding technique to temporarily form spatially and geometrically defined 3D cell aggregates in degradable scaffolds before transferring and culturing them in a reconstituted extracellular matrix. Herein, we demonstrate that Sacrificial Micromolding (1) promotes cyst formation and proper polarization of established epithelial cell lines, (2) allows reconstitution of heterotypic cell-cell interactions in multicomponent epithelia, and (3) can be used to control the lumenization-state of epithelial cysts as a function of tissue size. In addition, we discuss the potential of Sacrificial Micromolding as a cell-patterning tool for future studies.
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
Funders: U.S. Department of Defense, National Institutes of Health, University of California, San Francisco, National Defense Science and Engineering Graduate, National Institute of General Medical Sciences, National Center for Chronic Disease Prevention and Health Promotion, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Congressionally Directed Medical Research Programs
Awards: DECONSTRUCTING THE CELLULAR INTERACTIONS WITHIN THE BREAST CANCER MICROENVIRONMENT, Exploring Design Principles of Cellular Control Circuits, G2861609777, Cell Biology, Genetics, and Biochemistry Training Grant, Total synthesis of the human mammary gland, G4119534169, Medical Scientist Training Program, BC123047 A MULTISCALE AND MULTICOMPONENT IN VITRO MODEL OF THE HUMAN MAMMARY GLAND, Research Training in Chemistry and Chemical Biology, G4985329915, G5850682332, A Multiscale and Multicomponent in Vitro Model of the Human Mammary Gland, G8632625050, G9000218453
OpenAlex ID: W1835381180