Work

Analysis of cell-based RNAi screens

Year: 2006

Type: article

Abstract: RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (http://www.dkfz.de/signaling/cellHTS). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.

Source: Genome biology

DOI: 10.1186/gb-2006-7-7-r66

Authors: Michael Boutros, Lígia P Brás, Wolfgang Huber

Institutions: German Cancer Research Center, European Bioinformatics Institute, Instituto Superior Técnico, Universidade Técnica de Lisboa

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $5,490

OpenAlex ID: W1836834096

FWCI: 4.311

Cites: 39

Cited by: 305

Related to: 10