Work

Effect of Callus Induction Media on Morphology of Embryogenic Calli in Rice Genotypes

Year: 2002

Type: article

Abstract: Effects of four culture media on callus induction, regeneration and number of plants per unit culture were studied with mature seeds from five indica rice genotypes as explants. Based on the morphology, the calli were classified into four types as I to IV. Type I and type II are most suited to initiate suspension cultures or as target material for transformation. Number of plants regenerated per unit culture, formation of easily dissociating cell clusters and frequency of type I and type II calli were highest on NBKNB medium. Thus NBKNB medium is suitable for in vitro culture of even the hitherto recalcitrant indica genotypes.

Source: Biologia Plantarum

DOI: 10.1023/a:1022323221513

Authors: K.B.R.S. Visarada, M. Sailaja, N.P. Sarma

Institutions: Indian Institute of Millets Research, Indian Institute of Rice Research

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

OpenAlex ID: W18482522