Work

Strontium Phosphate Transfection of Human Cells in Primary Culture: Stable Expression of the Simian Virus 40 Large-T-Antigen Gene in Primary Human Bronchial Epithelial Cells

Year: 1987

Type: article

Abstract: Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.7.5.2031

Authors: D E Brash, R R Reddel, M Quanrud, K Yang, M P Farrell, C C Harris

Institutions: National Institutes of Health, National Cancer Institute, Georgetown University Medical Center, Georgetown Lombardi Comprehensive Cancer Center

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute