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Spine Formation and Correlated Assembly of Presynaptic and Postsynaptic Molecules

Year: 2001

Type: article

Abstract: Hippocampal pyramidal neurons in culture showed a developmental shift in synapse distribution from dendritic shafts to spines. Using dual wavelength time-lapse fluorescence microscopy, we analyzed the morphogenesis of three synaptic components: dendritic spines, postsynaptic densities (PSDs), and presynaptic vesicles. Local assembly of a major PSD protein, PSD-95, was spatially and temporally correlated with spine morphogenesis. Clustering of postsynaptic PSD-95 and that of a predominant synaptic vesicle protein, synaptophysin, were also correlated. In contrast, pre-existing PSD-95 clusters in dendritic shafts were preferentially eliminated without promoting spine formation. The local and stepwise assembly of synaptic components at the contact sites between dendritic protrusions and axons explains the developmental remodeling of excitatory synapses.

Source: Journal of Neuroscience

DOI: 10.1523/jneurosci.21-16-06105.2001

Authors: Shigeo Okabe, Akiko Miwa, Haruo Okado

Institutions: Tokyo Medical and Dental University, Japan Science and Technology Agency

Language: English

Topic: Neuroscience and Neuropharmacology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: green

Funders: Japan Science and Technology Corporation, Japan Science and Technology Agency, Core Research for Evolutional Science and Technology

OpenAlex ID: W1855713874

FWCI: 5.162

Cites: 35

Cited by: 298

Related to: 10