Year: 2010
Type: article
Abstract: Phenethyl isothiocyanate (PEITC), a natural compound found abundantly incruciferous and other vegetables, has been shown to possess cancer chemopreventive activity.The purpose of this investigation was to examine the cytotoxic effect of PEITC incholangiocarcinoma cells (CCA). Cholangiocarcinoma cells, KKU-100 and human liverChang cells used for comparison in the study. Effects of PEITC on cell growth and inductionof apoptosis was determined by fluorescent dye staining using acridine orange and ethidiumbromide. Cultured cells were exposed to PEITC for 3, 12, 24 and 48 hours followingassessment of cell viability and apoptotic cell death. PEITC can induce a large proportion ofcells to undergo apoptosis in a dose-time dependent manners. The PEITC induced depletionof intracellular antioxidant GSH in the cell lines. Moreover, a rapid collapse of themitochondrial transmembrane potential, as measured by JC-1 staining, was observedconcurrently with an apparent apoptosis in both cells. Furthermore, Western blot analysiswere used to examine the antioxidant and survival response related proteins. The resultsrevealed that PEITC increased levels of Nrf2 and cyclin D1 in both cell lines, and Bax andTrx protein expression was up-regulated in KKU-100. The effect of PEITC on cell growthand apoptosis may contribute to cancer chemopreventive properties. In conclusion, our datalucidly evidence the chemopreventive merits of dietary phytochemical PEITC in suppressionof cholangiocarcinoma.
Institution: Khon Kaen University
Language: Thai
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W1870808670