Exploring Cellular Targets of Vanillin Based on Morphological Changes of Saccharomyces cerevisiae
Type: dissertation
Abstract: Vanillin is one of the major phenolic compounds degraded from lignin.It is considered as a problematic byproduct of bioethanol production from lignocelluloses since it inhibits yeast growth and fermentation.However, detailed inhibitory mechanisms of vanillin are still unknown.In this study, I investigated intercellular targets of vanillin based on the image profiling method to infer the drug targets developed recently (Ohnuki et al., 2010).Using this method, I revealed that the morphology of wild-type cells treated with vanillin is similar with that of erg6Δ and his3Δ adh6Δ treated with vanillin.From the result of erg6Δ, the mutant of elgosterol-biosynthesis, it was suggested that vanillin does not affect its pathway.From the result of his3Δ adh6Δ, the mutant of vanillin-conversion, it was suggested that the accuracy of the image profiling was increased probably due to the inability of vanillin bio-conversion of his3Δ adh6Δ, which makes the effects of vanillin more aggravate.In common with the three strains, cell sizes were reduced in the presence of vanillin in a dose-dependent manner and significant enrichment of gene ontology term "ribosomal large subunit" was detected in the inference results.In conclusion, these results suggest that vanillin affects ribosome or related components in yeast cells and induces growth defect.
Author: Suga, Yohei
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W188460829