Work

Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli

Year: 1998

Type: article

Abstract: Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable.

Source: Journal of Bacteriology

DOI: 10.1128/jb.180.8.2063-2071.1998

Author: Kenan C. Murphy

Institution: University of Massachusetts Chan Medical School

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Institute of Allergy and Infectious Diseases

Award: BACTERIOPHAGE P22 ESSENTIAL RECOMBINATION FUNCTION