Work

Episomal Maintenance of a Bovine Papilloma Virus Vector in Transgenic Mice

Year: 1987

Type: article

Abstract: We have used a bovine papillomavirus-based vector to generate transgenic mice. Transgenic mice result from either pronuclear or cytoplasmic injections of the vector into fertilized eggs. Of 30 mice generated by microinjection, 27 (90%) contained the vector in its episomal form, at less than one copy per cell. This represents a highly efficient means of gene transfer in which the transgene is in a controlled genetic environment.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.7.3.1276

Authors: A Elbrecht, F J DeMayo, M J Tsai, B W O'Malley

Institutions: Texas Medical Center, Baylor College of Medicine

Language: English

Topic: Animal Genetics and Reproduction

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Medical Research Council Canada, National Institutes of Health, Medical Research Council, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Awards: G588632270, G9000294592