Work

Layer‐by‐layer inorganic/polymeric nanoparticles for kinetically controlled multigene delivery

Year: 2015

Type: article

Abstract: Nonviral gene delivery methods represent a potential safe and effective approach for treating myriad diseases. For many gene therapy applications, delivering multiple exogenous genes and controlling the time profile that these genes are expressed would be advantageous. Polymeric nonviral gene carriers are versatile and can be readily tailored for particular therapeutic applications, have the ability to carry multiple large genes within each particle, and can be more easily manufactured than viruses used for gene delivery. A layer-by-layer (LbL) theranostic-enabling nanoparticle was developed to incorporate two plasmid types which have differing expression time profiles. Temporally controlling the expression of exogenous DNA enables superior control over the microenvironment and could lead to better control over differentiation pathways and cell fate. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 707-713, 2016.

Source: Journal of Biomedical Materials Research Part A

DOI: 10.1002/jbm.a.35610

Authors: Corey J. Bishop, Allen L. Liu, David S. Lee, Richard J. Murdock, Jordan J. Green

Institutions: Johns Hopkins University, Johns Hopkins Medicine

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Eye Institute, National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering