Chemical Physiology of the Compound Eye, II.：Demonstration of Adrenaline from the Eye (With 1 Text-figure)
Year: 1963
Type: article
Abstract: Apparent evidence of sympathomimetic catechol amines was first adduced from insects by Ostlund (1953).This work formed a valuable contribution in dispelling some doubtful points in the past concerning the existence of the amines in insects.However, the detailed localization of such biogenic amines within insect bodies has remained an unsolved problem.In his previous papers (1962a, b), the present writer suggested the presence of adrenaline in rhabdom of the compound eyes of the house fly, Musca domestica vicina Macquart from its histochemical features.This paper deals with paper chromatographic and biologic demonstrations of adrenaline from the insect compound eye. Material and methodsMaterial: Females of the false stable fly, Muscina stabulans Fallen were mainly used in this study.They were reared in the laboratory on the same medium as in Musca domestica vicina, namely, a mixture consisting of sawdust, bean refuse (Okara) and entrails of fish.Sucrose and tap water were supplied to emerged adults.Procedure for dark and light adaptation: Prior to experiments for dark adaptation, adult specimens were overnighted in darkness without food.Light adapted specimens were obtained by irradiating overnight adults for 2-4 hours.In order to avoid the influence of their body movements in experiments for light adaptation, adult flies were irradiated after their wings and legs were firmly plastered with cellotape.Irradiation was made by means of a mercury vapour lamp (Toshiba Electric Inc., SLH-I00 UV), and a water vat (14.5 cm in thickness) was interposed between specimens and the lamp to absorb excess heat.Specimens subjected to irradiation were placed just behind the vat: where the brightness was ca.7600 lux and the rise of temperature was ca.4°C after 4 hour irradiation.
Author: Kenji Suzuki
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W189897621