Expression profiling of Aldh1l1‐precursors in the developing spinal cord reveals glial lineage‐specific genes and direct Sox9‐Nfe2l1 interactions
Year: 2013
Type: article
Abstract: Developmental regulation of gliogenesis in the mammalian CNS is incompletely understood, in part due to a limited repertoire of lineage-specific genes. We used Aldh1l1-GFP as a marker for gliogenic radial glia and later-stage precursors of developing astrocytes and performed gene expression profiling of these cells. We then used this dataset to identify candidate transcription factors that may serve as glial markers or regulators of glial fate. Our analysis generated a database of developmental stage-related markers of Aldh1l1+ cells between murine embryonic day 13.5-18.5. Using these data we identify the bZIP transcription factor Nfe2l1 and demonstrate that it promotes glial fate under direct Sox9 regulatory control. Thus, this dataset represents a resource for identifying novel regulators of glial development.
Source: Glia
DOI: 10.1002/glia.22538
Language: English
Subfield: Developmental Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: bronze
Funders: Howard Hughes Medical Institute, National Multiple Sclerosis Society, Cancer Prevention and Research Institute of Texas, Sandler Foundation, Multiple Sclerosis Society, National Institutes of Health, University of California, San Francisco, National Heart, Lung, and Blood Institute, National Institute of Mental Health, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Neurological Disorders and Stroke
Awards: Cellular and Genetic Origins of Astrocytes, Investigating Astrocyte Diversity and Function in Midbrain Dopaminergic Circuits, G3887021838, Medical Scientist Training Program, Neurobiological Mechanisms Underlying the Symptoms and Course of Schizophrenia, Transcriptional Control of Gliogenesis in the CNS, Research Training Award, Training In Cell and Gene Therapy, TRAINING THE NEXT GENERATION OF MENTAL HEALTH RESEARCHERS, Baylor College of Medicine Cancer Center - Cancer Center Support Grant
OpenAlex ID: W1899419375