Work

Cryopreservation of Strawberry Shoot Tips by Encapsulation-dehydration

Year: 2006

Type: article

Abstract: In vitro grown strawberry (Fragaria x ananassa Duch.) shoot tips were successfully cryopreserved using an encapsulation-dehydration procedure. Encapsulated shoot tips were precultured on MS medium supplemented with different sucrose concentrations (0.25, 0.5, 0.75 and 1.0 M) for 24 h and dehydrated (1 to 5 hours) in a sterile laminar air flow prior to direct immersion in liquid nitrogen (-196degree). A maximum of 63% regrowth of cryopreserved shoot tips was obtained following preculture in 0.75 M sucrose solution and 4 h dehydration. Plants produced from control and cryopreserved shoot tips were phenotypically similar.

Source: DOAJ (DOAJ: Directory of Open Access Journals)

DOI: 10.15835/nbha341260

Authors: Adela Halmágyi, Constantin Deliu

Institutions: Institutul de Cercetari Biologice Cluj-Napoca, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

OpenAlex ID: W1902632765

FWCI: 0.426

Cites: 8

Cited by: 7

Related to: 10