The influence of donor and recipient strains in isolated small bowel transplantation in rats.
Year: 1994
Type: article
Abstract: E recently reported that intestinal transplantation from Lewis (LEW) to Brown Norway (BN) was intractable to FK 506 therapy because of a high incidence of a graft-versus-host disease (GVHD) syndrome) similar to that in the parent to F) hybrid offspring experiments of Monchik and Russell. 2 However, transplantation in the opposite direction (BN to LEW) was highly successful without either GVHD or difficult rejection reactions.Genetic analysis did not provide an explanation for the directional difference, in spite of the fact that genetic factors are known to control alloreactions. 3 .4 Consequently, we undertook the experiments herein reported to determine the influence on intestinal transplant outcome of 12 donorrecipient combinations of 4 fully allogeneic rat strains, with or without a 2-week induction course of FK 506.The outcome indices of rejection and GVHD proved to be influenced overwhelmingly by the choice of the recipient strain, with the choice of the donor being a far less consequential factor. MATERIALS AND METHODS Intestinal TransplantationAnimals.Inbred male LEW (RTlI), BN (RTl").ACI (RTla).and PY G (R Tl C) rats weighing 200 to 300 g were purchased from Harlan Sprague Dawley Inc (Indianapolis, Ind) and used as donors or recipients.All animals were kept in conventional facilities.Water and commercial rat chow were provided ad libitum.Intestinal Transplant Operation.Under methoxyflurane anesthesia.orthotopic small bowel transplantation (SBTx) was performed as described before.5 For convenience, venous drainage was into the recipient inferior vena cava, a procedure that has been shown in our laboratories 6 and elsewhere 7 not to affect the immune reactions.The entire small intestine from the ligament of Trietz to the ileocecal valve was harvested on a vascular pedicle, consisting of the superior mesenteric artery with a piece of aorta and portal vein.End-to-side vascular anastomoses hetween the graft aorta and recipient infrarenal aorta.and between the graft portal vein and recipient vena cava, were hand sewn with 10-0 Novafil suture.The entire recipient intestine was removed and enteric continuity was restored via proximal and distal end-to-end intestinal anastomoses.Immunosuppression.FK 506 was dissolved in HCO-60 and Dmannitol carrier solvent and was given intramuscularly at a dose of 0.64 mg/kgld for 14 days starting on the day of intestlflal transplantation.Body weight, activity, skin color.and defecation patterns were monitored daily for the first 14 days, and at least twice a week thereafter for 150 days.Clinical signs of rejection were defined as continuous or intermittent diarrhea and weight loss.GVHD was clinically defined by cutaneous erythema.and by hair and weight loss. Tissue Collection.A complete necropsy was performed on the animals that died before 150 days.Pieces of tissue from graft From the Pittsburgh Transplant Institute (M.
Source: PubMed
Authors: M Tanabe, N Murase, A J Demetris, R A Hoffman, K Nakamura, S Fujisaki, F H Galvao, S Todo, J Fung, T E Starzl
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funder: National Institutes of Health
OpenAlex ID: W190602167