Work

FRET sensor-based quantification of intracellular trehalose in mammalian cells

Year: 2015

Type: article

Abstract: Trehalose acts as a stress protectant and an autophagy inducer in mammalian cells. The molecular mechanisms of action remain obscure because intracellular trehalose at micromolar level is difficult to quantitate. Here, we show a novel trehalose monitoring technology based on FRET. FLIP-suc90μ∆1Venus sensor expressed in mammalian cells enables to quickly and non-destructively detect an infinitesimal amount of intracellular trehalose.

Source: Bioscience Biotechnology and Biochemistry

DOI: 10.1080/09168451.2015.1069699

Authors: Shingo Kikuta, Bi-Huei Hou, Ryoichi Sato, Wolf B Frommer, Takahiro Kikawada

Institutions: Institute of Agrobiological Sciences, Tokyo University of Agriculture and Technology, Carnegie Institution for Science, Carnegie Department of Plant Biology

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze