Year: 2007
Type: dissertation
Abstract: We evaluated the abilities of the Vircell immunoglobulin G (IgG) and IgM indirect immunofluorescence assay (IFA) for Legionella pneumophila serogroup 1, the IgM and IgG enzyme-linked immunosorbent assay (ELISA) for Legionella pneumophila serogroup 1, and the IgM-plus-IgG ELISA for Legionella pneumophila serogroups 1 to 6 to diagnose Legionnaires’ disease (LD) in a sample of patients with and without LD. Also, we determined the agreements, sensitivities, and specificities of the different Vircell assays in comparison to a validated ELISA (Serion classic ELISA). Clinical sensitivity and specificity were 74.6% and 96.6%, respectively, for the IgM IFA, 65.1% and 88.0% for the IgG IFA, 92.3% and 100% for the IgM ELISA, 43.3% and 96.6% for the IgG ELISA, and 90.8% and 100% for the IgM-plus-IgG ELISA. Compared to Serion classic ELISA, agreement, sensitivity, and specificity were 79.4%, 66.2%, and 88.9%, respectively, for the IgM IFA, 75.0%, 59.3%, and 83.8% for the IgG IFA, 89.5%, 97.3%, and 83.3%, respectively, for the Vircell IgM ELISA, 81.9%, 55.2%, and 96.3%, respectively, for the Vircell IgG ELISA, and 93.5%, 96.0%, and 91.5%, respectively, for the Vircell IgM and IgG combined ELISA. The value of a positive diagnostic result obtained by the Vircell IgM IFA, the Vircell IgG IFA, and the Vircell IgG ELISA might not be acceptable for a diagnostic assay. Both the high specificities and sensitivities of the Vircell IgM ELISA and the IgM-plus-IgG ELISA and the high correlation with the Serion classic ELISA indicate that they are useful in the diagnosis of LD. Evaluation of VIRCELL Enzyme Immunoassay and Indirect Immunofluorescent Assay 31
Author: B. M. W. Diederen
Language: English
Subfield: Endocrinology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1912034485