Year: 2015
Type: article
Abstract: Triploid grapes were produced by in ovulo embryo culture. Ovules were obtained from 5 crosses: Rosario Bianco (V. vinifera L. 2x) x Kyoho (V. vinifera x V. labrusca L. 4x), Katta Kourgan(V. vinifera L. 2x)x Kyoho, Kyoho x Rosario Bianco, Kyoho x Sekirei (V. vinifera L. 2x) and Kyoho x Rizamat (V. vinifera L. 2x). Most embryos were obtained from ovules cultured at veraison. The addition of indole-3-acetic acid (2.0 mg·l-1) and gibberellic acid (0.4 mg·l-1 to 1/2 MS (half strength macro-elements) medium promoted the formation and growth of embryos. Although only a few embryos germinated directly from ruptured ovules, embryos excised from cultured ovules germinated successfully by in vitro culture. This paper demonstrates that in ovulo embryo culture is expected to be an efficient method to produce triploid grapes.
Source: Vitis
Authors: Yamashita, H., Shigehara, I., Haniuda, T.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W1914595232