STRUCTURAL FEATURES OF THE 5' -FLANKING REGION OF THE β-LACTO GLOBULIN GENE OF BUFFALO (BUBALUS BUBALIS)
Year: 2009
Type: article
Abstract: β-lactoglobulin is an abundant globular milkwhey protein expressed in glandular epithelium of the mammary gland of ruminants and some other species. The expression of β-lactoglobulin is controlled by different regulatory elements which act through its promoter. The present study was designed to clone and characterize the sequence of promoter region of β-lactoglobulin gene. The genomic DNA was isolated from buffalo white blood cells (WBCs). The promoter region of β-lactoglobulin gene was amplified using PCR, then cloned and sequenced. The sequence has been submitted to the nucleotide database of the National Centre for Biological Information (NCBI)/GenBank with the accession number AM 238696. Computational analysis of the sequence showed the presence of highly conserved core putative transcription factors (presented in the TRANSFAC database) binding sites, viz., signal transduction and activator of transcription (STAT V), insulin response element, glucocorticoid response elements, pregnancy specific mammary nuclear factor, yin and yang factor (YY1) and CCAAT/enhancer binding protein (C/EBP). Homologies of 86.4, 75.1, and 80.3 %, were found with bovine, sheep, and goat, respectively. A phylogenetic tree constructed on the basis of sequence comparisons showed that the buffalo is evolutionarily more closely related to cattle with respect to this gene.
Source: Buffalo Bulletin
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W1914813747