Year: 2001
Type: article
Abstract: Rapid method of in vitro multiplication of date palm was developed. Shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with 2 mg dm-3 dimethylaminopurine (2iP) + 1 mg dm-3 naphthalene acetic acid (NAA). Shoot buds were proliferated from white nodular cultures on hormone free medium. Shoot bud proliferation strongly enhanced when cultured on MS-medium contained 3 mg dm-3 2iP + 0.5 mg dm-3 NAA. Culturing on full-strength MS medium showed higher multiplication rate compared with half-strength MS medium. Among four concentrations of sucrose used, 30 g dm-3 speeded up the bud proliferation more than 10, 20 and 40 g dm-3. However, the largest shoot buds were observed with 40 g dm-3 sucrose. Solidification of culture media by 1.75 g dm-3Phytagel showed the highest proliferation rate, but the largest buds were observed with 1 g dm-3Phytagel.
Source: Biologia Plantarum
Authors: H.S. Taha, S.A. Bekheet, M.M. Saker
Institution: National Research Centre
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W191560700