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Pseudomonas aeruginosa biofilm matrix polysaccharide Psl is regulated transcriptionally by RpoS and post‐transcriptionally by RsmA

Year: 2010
Type: article
Abstract: Extracellular polysaccharides are important components of biofilms. In non-mucoid Pseudomonas aeruginosa strains, the Pel and Psl polysaccharides are major structural components of the biofilm matrix. In this study, we demonstrate that the alternative σ-factor RpoS is a positive transcriptional regulator of psl gene expression. Furthermore, we show that psl mRNA has an extensive 5' untranslated region, to which the post-transcriptional regulator RsmA binds and represses psl translation. Our observations suggest that upon binding RsmA, the region spanning the ribosome binding site of psl mRNA folds into a secondary stem-loop structure that blocks the Shine-Dalgarno sequence, preventing ribosome access and protein translation. This constitutes a novel mechanism for translational repression by this family of regulators.
DOI: 10.1111/j.1365-2958.2010.07320.x
Authors: Yasuhiko Irie, Melissa Starkey, Adrianne N. Edwards, Daniel J. Wozniak, Tony Romeo, Matthew R. Parsek
Institutions: University of Washington, University of Iowa, Harvard University, Massachusetts General Hospital, Emory University, The Ohio State University, University of Florida
Language: English
Open Access status: bronze
Funders: National Science Foundation, U.S. Department of Agriculture, National Institutes of Health, U.S. Public Health Service, National Heart, Lung, and Blood Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases
Awards: Biofilm Formation and Dispersal Mechanisms, The matrix of Pseudomonas aeruginosa biofilms, G5343506360, Pel Polysaccharide of Pseudomonas Aeruginosa, The matrix of Pseudomonas aeruginosa biofilms, The pel exopolysaccharide gene cluster of Pseudomonas aeruginosa, Mechanism of CsrA-Mediated Global Control, The matrix of Pseudomonas aeruginosa biofilms, Control of P. aeruginosa algD transcription, G7531984491
OpenAlex ID: W1918000672
FWCI: 5.101
Cites: 74
Cited by: 283
Related to: 10