Multiplexed detection of viral infections using rapid in situ RNA analysis on a chip
Year: 2015
Type: article
Abstract: Viral infections are a major cause of human disease, but many require molecular assays for conclusive diagnosis. Current assays typically rely on RT-PCR or ELISA; however, these tests often have limited speed, sensitivity or specificity. Here, we demonstrate that rapid RNA FISH is a viable alternative method that could improve upon these limitations. We describe a platform beginning with software to generate RNA FISH probes both for distinguishing related strains of virus (even those different by a single base) and for capturing large numbers of strains simultaneously. Next, we present a simple fluidic device for reliably performing RNA FISH assays in an automated fashion. Finally, we describe an automated image processing pipeline to robustly identify uninfected and infected samples. Together, our results establish RNA FISH as a methodology with potential for viral point-of-care diagnostics.
Source: Lab on a Chip
DOI: 10.1039/c5lc00459d
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
Funders: National Science Foundation, National Institutes of Health, Agency for Healthcare Research and Quality, U.S. Public Health Service, NIH Office of the Director, National Cancer Institute, National Institute of Allergy and Infectious Diseases, National Institute of Biomedical Imaging and Bioengineering
Awards: G1116296131, G1214792496, NSEC on Molecular Function at the Nano/Bio Interface, G4172110146, HEALTHCARE-ASSOCIATED RESPIRATORY INFECTION IN THE PEDIATRIC AMBULATORY SETTING, G4362848896, G4478190008, A micro Hall chip for circulating microvesicle based cancer monitoring, Impact of prior influenza exposures on antibody repertoires to new viral strains, A Comprehensive Spatial Picture of Transcription in the Nucleus, Validation and development of single nucleotide variant RNA FISH in single cells in culture and tissue, G6793692892, G8252985069, G8315325558, CAREER: Unraveling homeostatic mechanisms in gene expression regulation: Integrating research and scientific communication, G9000170754, Consolidated Basic Cancer Research Program
OpenAlex ID: W1918979484