Work

Adenovirus E1B Proteins Are Required for Accumulation of Late Viral mRNA and for Effects on Cellular mRNA Translation and Transport

Year: 1985

Type: article

Abstract: Late in adenovirus infection, large amounts of viral mRNA accumulate while cell mRNA transport and translation decrease. Viruses deleted in the E1B region of type 5 adenovirus do not produce the same outcome: (i) viral mRNA synthesis by the mutants is normal, delivery to the cytoplasm is 50 to 75% of normal, but steady-state levels of viral mRNA are decreased 10-fold; (ii) cell mRNA synthesis and transport continue normally in the mutant virus-infected cell; and (iii) translation of preexisting cell mRNA which is disrupted in wild-type infection remains normal in mutant-virus-infected cells. Thus E1B proteins are required for accumulation of virus mRNA and for induction of the failure of host cell mRNA transport and translation. If a single function is involved, by inference the transport and some aspect of translation of mRNAs could be linked.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.5.10.2552-2558.1985

Authors: L E Babiss, H S Ginsberg, J E Darnell

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Cancer Institute, National Institute of Allergy and Infectious Diseases

Awards: G236222578, MOLECULAR PATHOGENESIS OF ADENO DISEASES--ANIMAL MODELS