Work

A Three-Megabase Yeast Artificial Chromosome Contig Spanning the C57BL Mouse Igh Locus

Year: 2002

Type: article

Abstract: The mouse Ig H chain (Igh) complex locus is composed of >100 gene segments encoding the variable, diversity, joining, and constant portions of the Ab H chain protein. To advance the characterization of this locus and to identify all the V(H) genes, we have isolated the entire region from C57BL/6 and C57BL/10 as a yeast artificial chromosome contig. The mouse Igh locus extends approximately three megabases and contains at least 134 V(H) genes classified in 15 partially interspersed families. Two non-Igh pseudogenes (Odc-rs8 and Rpl32-rs14) were localized in the distal part of the locus. This physical yeast artificial chromosome map will provide important structure and guidance for the sequencing of this large, complex, and highly repetitive locus.

Source: The Journal of Immunology

DOI: 10.4049/jimmunol.168.11.5659

Authors: Christophe Chevillard, Jennifer Ozaki, Christopher D Herring, Roy Riblet

Institutions: Inserm, Vanderbilt University, University of Wisconsin–Madison, Torrey Pines Institute For Molecular Studies

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Awards: G1597933449, Genetic Analysis of the Immune System