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Aldosterone-stimulated sodium uptake by apical membrane vesicles from A6 cells.

Year: 1984

Type: article

Abstract: Sodium fluxes in plasma membrane vesicles prepared from the cultured toad kidney epithelial cell line A6 are studied. The vesicles are enriched 7-10 times in apical membrane markers. Sodium uptake is osmotically sensitive and inhibited by low concentrations of amiloride (K0.5 = 7 X 10(-8) M at 1 mM NaCl). Vesicles prepared from aldosterone-treated cells (4.5 h at 10(-7) M aldosterone) show a 2-fold enhancement of amiloride-sensitive sodium flux relative to appropriate controls. The above observations are in good agreement with studies of sodium transport across the apical membrane of intact A6 epithelia. Thus, the amiloride-sensitive sodium transporter in the apical membrane of these cells is preserved in the vesicle preparation, making it possible to study the effects of aldosterone in the absence of nonmembrane-related phenomena.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(18)90850-1

Authors: S Sariban-Sohraby, M B Burg, R J Turner

Institutions: National Institutes of Health, Harvard University, University of Toronto, National Heart, Lung, and Blood Institute

Language: English

Topic: Ion Transport and Channel Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W1926935438

FWCI: 2.127

Cites: 15

Cited by: 44

Related to: 10