Properti Probiotik Isolat Bakteri Asam Laktat untuk Mengendalikan Pertumbuhan Vibrio alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus)
Year: 2012
Type: article
Abstract: Tujuan penelitian ini adalah untuk menyeleksi isolat bakteri asam laktat (BAL) yang telah diisolasi dari ikan Kerapu Macan ( Epinephelus fuscoguttatus ) berdasarkan toleransi terhadap pH dan bile salts, patogenisitas dan kemampuannya menekan pertumbuhan Vibrio alginolyticus pada uji ko-kultur. Hasil penelitian menunjukkan bahwa dua puluh isolat BAL yang telah diuji antagonismenya terhadap V. alginolyticus pada penelitian terdahulu, dapat hidup pada pH rendah 3 dan tumbuh pada konsentrasi bile salts 0,5% kecuali isolat KSBU 13D. Semua isolat tidak patogen terhadap ikan Kerapu Macan setelah diinfeksi BAL pada konsentrasi 10 7 cfu/ml secara intraperitoneal (IP). Pada uji ko-kultur, dari 6 isolat BAL yang dipilih, tiga isolat (KSBU 12C, KSBU 5Da, KSBU 9) berturut-turut mampu menekan pertumbuhan V. alginolyticus , dan penambahan konsentrasi BAL terbaik adalah 10 7 cfu/ml. Kata kunci: bakteri asam laktat, vibriosis, patogenisitas, ko-kultur, kerapu macan The research objective was to select lactic acid bacteria which previously isolated from Tiger Grouper ( Epinephelus fuscoguttatus ) based on pH and bile salts tolerances, pathogenicity test and ability to decrease the growth of Vibrio alginolyticus in co-culture test. The result shows that twenty isolates which had also been examined their antagonisms against V. alginolyticus previously, were able to survive relatively low pH values 3 and able to survive at high bile salts concentrations 0.5% except KSBU 13D isolate. All isolates were nonpathogenic to Tiger Grouper after being intraperitoneally infected with the lactic acid bacteria at concentration 10 7 cfu/ml. In co-culture test, V. alginolyticus was reduced significantly by three isolates (KSBU 12C, KSBU 5Da, KSBU 9) from 6 isolates selected, and the best additional bacterial concentration was 10 7 cfu/ml. Key words: lactic acid bacteria, vibriosis, pathogenicity, kerapu macan
Institutions: Riau University, Universitas Gadjah Mada
Language: Indonesian
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $175
OpenAlex ID: W1927951411