The Isolation and Characterization of Subcellular Components of the Epithelial Cells of Rabbit Small Intestine
Year: 1965
Type: article
Abstract: 1. Homogenization of the epithelial cells of rabbit small intestine in 0.3m-sucrose-5mm-EDTA, pH7.4, maintains intact the microvillus sheets that form the lumenal surface of the cells, the nuclei, the mitochondria and the vesicles (microsomes) formed from the endoplasmic reticulum. 2. These particulate components of the cell, and the cell-sap fraction, have been isolated by differential centrifuging of cell homogenates. 3. The nuclei and microvillus sheets sediment together and it has been impossible to separate these subcellular components by centrifugal methods. 4. The isolated subcellular fractions have been identified by a combination of light-microscopic examination, electron-microscopic examination, chemical analysis and assay for selected enzyme activities.
Source: Biochemical Journal
DOI: 10.1042/bj0960159
Authors: JW PORTEOUS, B CLARK
Institution: University of Aberdeen
Language: English
Subfield: Gastroenterology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Funder: Medical Research Council
OpenAlex ID: W1933968888