Work

High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis

Year: 2013

Type: book-chapter

Abstract: Invadopodia are specialized structures of cancer cells which aid in cancer cell invasion and metastasis. Therefore, studying the early steps of invadopodium assembly and its life cycle at the subcellular level by using high spatiotemporal resolution imaging provides an opportunity for understanding the signaling mechanisms involved in this very important process. In this chapter, we describe the design of a custom-built high-resolution fluorescence microscope which makes this challenging imaging possible. We also describe an ImageJ plugin that we have developed for tracking of invadopodia and lifetime analysis.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-538-5_21

Authors: Ved P. Sharma, David Entenberg, John Condeelis

Institution: Albert Einstein College of Medicine

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Awards: Tumor microenvironments determing migration, dissemination and dormancy, Signal transduction regulating tumor cell dissemination, G8076743859