Work

Use of 31PNMR spectroscopy to follow the time course of phosphatidylcholine chemical synthesis.

Year: 1985

Type: article

Abstract: 31PNMR spectroscopy is shown to be useful for studying the chemical synthesis of phosphatidylcholine from phosphatidic acid and choline. Sharp, well-resolved resonances were obtained by chelating multivalent cations, thereby enabling quantitation of reactants, products, and intermediates. The syntheses of several types of phosphatidylcholines were monitored by 31PNMR spectroscopy, including perdeuterated and headgroup spin-labeled molecules. For perdeuterated phosphatidylcholines, this analysis led to reaction conditions giving much better conversion to product than conditions previously observed. In addition, a polyphosphate side-product was identified in reactions which do not produce phosphatidylcholine, implying either a polyphosphate intermediate in the reaction mechanism, or else a competing side reaction.

Source: Journal of Lipid Research

DOI: 10.1016/s0022-2275(20)34319-4

Authors: P R Meers, G W Feigenson

Institution: Cornell University

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of General Medical Sciences