Work

Bessel-beam Grueneisen relaxation photoacoustic microscopy with extended depth of field

Year: 2015

Type: article

Abstract: The short focal depth of a Gaussian beam limits the volumetric imaging speed of optical resolution photoacoustic microscopy (OR-PAM). A Bessel beam, which is diffraction free, provides a long focal depth, but its side lobes deteriorate image quality when the Bessel beam is directly employed to excite photoacoustic (PA) signals in OR-PAM. We present a nonlinear approach based on the Grueneisen relaxation effect to suppress the side-lobe artifacts in PA imaging. This method extends the focal depth of OR-PAM and speeds up volumetric imaging. We experimentally demonstrated a 1-mm focal depth with a 7-μm lateral resolution and volumetrically imaged a carbon fiber and red blood cell samples.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.jbo.20.11.116002

Authors: Junhui Shi, Lidai Wang, Cedric Noordam, Lihong V. Wang

Institutions: Washington University in St. Louis, City University of Hong Kong, University of Twente

Language: English

Topic: Photoacoustic and Ultrasonic Imaging

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering, National Center for Chronic Disease Prevention and Health Promotion

Awards: Photon tunneling: Shedding new light on biomedicine, G1952371934, In vivo imaging of single circulating cells, PHOTOACOUSTIC MICROSCOPY OF NEOVASCULARIZATION IN RENAL CANCER GROWTH AND THERAPY