microRNA‐dependent temporal gene expression in the ureteric bud epithelium during mammalian kidney development
Year: 2014
Type: article
Abstract: BACKGROUND: Our previous study on mouse mutants with the ureteric bud (UB) epithelium-specific Dicer deletion (Dicer UB mutants) demonstrated the significance of UB epithelium-derived miRNAs in UB development. RESULTS: Our whole-genome transcriptional profiling showed that the Dicer mutant UB epithelium abnormally retained transcriptional features of the early UB epithelium and failed to express many genes associated with collecting duct differentiation. Furthermore, we identified a temporal expression pattern of early UB genes during UB epithelium development in which gene expression was detected at early developmental stages and became undetectable by embryonic day 14.5. In contrast, expression of early UB genes persisted at later stages in the Dicer mutant UB epithelium and increased at early stages. Our bioinformatic analysis of the abnormally persistently expressed early genes in the Dicer mutant UB epithelium showed significant enrichment of the let-7 family miRNA targets. We further identified a temporal expression pattern of let-7 miRNAs in the UB epithelium that is anti-parallel to that of some early UB genes during kidney development. CONCLUSIONS: We propose a model in which the let-7 family miRNAs silence the expression of a subset of early genes in the UB epithelium at later developmental stages to promote collecting duct differentiation. Developmental Dynamics 244:444-456, 2015. © 2014 Wiley Periodicals, Inc.
Source: Developmental Dynamics
DOI: 10.1002/dvdy.24221
Language: English
Topic: Renal and related cancers
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Funders: American Heart Association, Medical University of South Carolina, Cincinnati Children's Hospital Medical Center, University of South Carolina, Georgia Clinical and Translational Science Alliance, National Heart and Lung Institute, National Institutes of Health, South Carolina Clinical and Translational Research Institute, Medical University of South Carolina, National Heart, Lung, and Blood Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Research Resources, National Center for Advancing Translational Sciences
Awards: South Carolina Clinical & Translational Research Institute (SCTR), G2935669560, G3211369444, G3314368649, Molecular and Cellular Regulation of Mammalian Renal Medulla Development, G3695625607, G3843897372, South Carolina Clinical & Translational Research Institute (SCTR), G5097421434, G5989007308, SC COBRE for Developmentally Based Cardiovascular Diseases, Mucopolysaccharide Metabolism in Cardiac Anomalies, G8536935163
OpenAlex ID: W1940829406