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Human Embryonic Kidney Cells: Stable Transformation with an Origin-Defective Simian Virus 40 DNA and Use as Hosts for Human Papovavirus Replication

Year: 1984

Type: article

Abstract: An origin-defective mutant DNA of simian virus 40 immortalized human embryonic kidney cells, maintaining a T protein which could function for human papovavirus BK DNA replication but not for human papovavirus JC DNA replication. Neither BK virions nor capsid proteins were produced in these cells. This may indicate that the simian virus 40 T protein in human embryonic kidney cells is competent for maintaining transformation and initiating and completing DNA replication for BK but is not competent for switching to late gene functions. Furthermore, it appears that the JC DNA replication origin cannot efficiently use the simian virus 40 T protein for its DNA synthesis, as suggested by its DNA sequence data (R. Frisque, J. Virol. 46:170-176, 1983; T. Miyamura, H. Jikoya, E. Soeda, and K. Yoshiike, J. Virol. 45:73-79, 1983).

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.4.2.379

Authors: E O Major, P Matsumura

Institutions: National Institutes of Health, University of Illinois Chicago, National Institute of Neurological Disorders and Stroke

Language: English

Topic: Polyomavirus and related diseases

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W1942228673

FWCI: 3.002

Cites: 17

Cited by: 37

Related to: 10