Development and Validation of Reversed Phase High Performance Liquid Chromatographic Method for Simultaneous Estimation of Paracetamol, Caffeine and Carisoprodol in Tablet Formulation
Year: 2015
Type: article
Abstract: Combined dose tablet formulation containing Paracetamol, Caffeine and Carisoprodol is used for thetreatment of low back pain, post traumatic muscle spasm, sprains, strains and tenosynovitis. In this studya simple, specific, precise and accurate reverse phase high performance liquid chromatographic (RPHPLC) methods has been developed for simultaneous estimation of paracetamol (PAR), caffeine (CAF)and carisoprodol (CAR) in tablet dosage form. In the proposed chromatographic method separation wasachieved by HiQ silC-18HS column (250 mm× 4.6 mm), with mobile phase containing Acetonitrile:Buffer (0.1mol L-1 Orthophosphoric Acid) (30:70 v/v) and the pH of the Buffer was adjusted to 3.1 bytriethylamine. The flow rate was 1.0 ml min-1and effluent was monitored at 232.2 nm. The retentiontime of CAF, PAR and CAR were 2.804 min, 4.815 min and 6.718 min respectively. The linearity forPAR, CAF and CAR were in the range of 5-25 µg mL-1, 5-25 µg mL-1and 10-50 µg mL-1respectively.The recoveries of PAR, CAF and CAR were found in the range of 99.05-99.78 %, 98.47-99.90 % and98.92-99.56 % respectively. The proposed methods were validated as per International Conference onHarmonisation (ICH) guidelines by means of different parameters likes linearity, precision, accuracy,and limit of detection, limit of quantitation, range and selectivity, robustness ruggedness, solutionstability as per ICH guidelines and successfully applied to the estimation of PAR, CAF and CAR in thetablet dosage form.
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W1948708601