Static Biofilm Cultures of Gram‐Positive Pathogens Grown in a Microtiter Format Used for Anti‐Biofilm Drug Discovery
Year: 2010
Type: article
Abstract: An in vitro assay is presented for culturing staphylococcal biofilms and biofilms of nonmotile Gram-positive bacteria under static conditions in microtiter assay plates, and for the quantification of biofilm growth, using a simple staining procedure that measures amounts of bacterial cells and extracellular matrix. This basic assay can be adapted readily to study several aspects of biofilm formation, for high-throughput screening to identify small molecule inhibitors of biofilm formation or biofilm-defective mutants, and for quantifying the anti-biofilm activity of biofilm inhibitors.
Authors: Steven M. Kwasny, Timothy J. Opperman
Institution: Microbiotix (United States)
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: c-diGMP Signal Transduction Proteins are Biofilm Drug Targets, THERAPEUTIC APPROACHES TO BIOFILM ANTIBIOTIC RESISTANCE, Novel Therapies for BioFilm-Related Infections, Inhibitors of wall teichoic acid biosynthesis of Staphylococcus aureus, A NOVEL BIS-INDOLE COMPOUND AGAINST CATHETER COLONIZATION, Antibiotic Potentiators Targeting SOS Induction
OpenAlex ID: W1949386595