A RAPID, EASY AND HIGH YIELD PROTOCOL FOR TOTAL GENOMIC DNA ISOLATION OF Colletotrichum gloeosporioides AND Fusarium oxysporum
Year: 2008
Type: article
Abstract: A rapid, easy and simple protocol for total genomic DNA isolation was developted for Colletotrichum gloeosporioides and Fusarium oxysporum . The total genomic DNA yield was very high and suitable to RAPD (random amplified polymorphic DNA), and restriction endonuclease reactions. DNA obtained by this protocol was not contaminated by protein or carbohydrate, and proteases, cesium chloride or phenol were also not necessary.
Source: Revista UNIMAR
Author: Eiko E. Kuramae
Institution: Universidade Estadual Paulista (Unesp)
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W1949597144
