Phospholipase A of Guinea Pig Spermatozoa: Its Preliminary Characterization and Possible Involvement in the Acrosome Reaction. (guinea pig/spermatozoa/acrosome reaction/phospholipase A)
Year: 1982
Type: article
Abstract: Phospholipase A activity was demonstrated in guinea pig spermatozoa using [U-14C] phosphatidyl choline as a substrate. The activity had a neutral pH optimum, was stimulated by Ca2+ and low concentrations of detergent, and. was inhibited by EDTA, mepacrine and p-bromophenacyl bromide. Appropriate concentrations of mepacrine and p-bromophenacyl bromide inhibited the acrosome reactions of capacitated spermatozoa without interfering with their motility. These results support the notion that phospholipase A is involved in the acrosome reaction of mammalian spermatozoa.
Institution: University of Hawaii System
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W1954874744