Plasmid-borne Tn5 insertion mutation resulting in accumulation of gentisate from salicylate
Year: 1985
Type: article
Abstract: Plasmid-borne Tn5 insertion mutants of a Pseudomonas species which accumulated 2,5-dihydroxybenzoate (gentisate) following growth on 2-hydroxybenzoate (salicylate) were obtained from a pool of mutants that were unable to grow on naphthalene. One such mutant was characterized further. The ability of this mutant to oxidize gentisate was 100-fold less than the ability of a Nah+ Sal+ strain harboring the unmutagenized plasmid, although both strains oxidized and grew on salicylate. These bacteria were presumably able to metabolize salicylate via catechol, since they possessed an inducible, plasmid-encoded catechol 2,3-dioxygenase. Our results suggest that there is an alternate, plasmid-encoded route of salicylate degradation via gentisate and that some plasmid-associated relationship between this pathway and naphthalene oxidation exists.
Authors: D J Monticello, D Bakker, M Schell, W R Finnerty
Language: English
Subfield: Pollution
Field: Environmental Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W1959842993