Work

Molecular cloning and nucleotide sequence of the streptavidin gene

Year: 1986

Type: article

Abstract: Using synthetic oligonucleotides as probes we have cloned the streptavidin gene from a genomic library of Streptomyces avidinii. Nucleotide sequence analysis indicated that a 2 Kb DNA-fragment contained the entire coding region, a signal peptide region and the 3' and 5' flanking regions of the gene. The deduced amino acid sequence shows several interrupted blocks of homology with the amino acid sequence of chicken egg-white avidin. Analysis of the secondary structure suggests a high content of beta-structure in both proteins and considerable overall structural similarity between them.

Source: Nucleic Acids Research

DOI: 10.1093/nar/14.4.1871

Authors: Carlos E. Argarana, Irwin D. Kuntz, Steven Birken, Richard Axel, Charles R. Cantor

Institutions: Columbia University, University of California, San Francisco

Language: English

Topic: Biotin and Related Studies

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Awards: G1024383398, G1385511475, G3698234551