480. Gene Transfer to Gut K Cells for Physiologic Insulin Replacement in Diabetes
Year: 2006
Type: article
Abstract: We have previously demonstrated the feasibility of using gut K- cells as surrogate cells for insulin production. Transgenic mice expressing human insulin specifically in K-cells remained normoglycemic and glucose tolerant following destruction of their pancreatic |[beta]|-cells. However, a vector system is needed to transfer effective amount of the therapeutic insulin gene to duodenal epithelial cells in vivo. Previous efforts using viral and non-viral gene delivery vector resulted in some success. However, persistent transgene expression and detectable circulating proteins were not observed. Accordingly, this study is to identify a suitable viral vector and an optimal delivery methodology to achieve long-term gene transfer to the duodenum.
Source: Molecular Therapy
Authors: Eric C. Hsu, Scott Pownall, Timothy J. Kieffer, Yvonne Luu, Simon Leong, Paul D. Robbins, Xiao Xiao, Anthony T. Cheung
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
OpenAlex ID: W1963969662