Work

Microbeam-integrated multiphoton imaging system

Year: 2008

Type: article

Abstract: Multiphoton microscopy has been added to the array of imaging techniques at the endstation for the Microbeam II cell irradiator at Columbia University's Radiological Research Accelerator Facility (RARAF). This three-dimensional (3D), laser-scanning microscope functions through multiphoton excitation, providing an enhanced imaging routine during radiation experiments with tissuelike samples, such as small living animals and organisms. Studies at RARAF focus on radiation effects; hence, this multiphoton microscope was designed to observe postirradiation cellular dynamics. This multiphoton microscope was custom designed into an existing Nikon Eclipse E600-FN research fluorescence microscope on the irradiation platform. Design details and biology applications using this enhanced 3D-imaging technique at RARAF are reviewed.

Source: Review of Scientific Instruments

DOI: 10.1063/1.3043439

Authors: Alan W. Bigelow, Charles R. Geard, Gerhard Randers-Pehrson, David J. Brenner

Institutions: Columbia University Irving Medical Center, Columbia University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Aeronautics and Space Administration, National Institute of Biomedical Imaging and Bioengineering

Awards: Radiological Research Accelerator Facility (RARAF), MECH. OCULAR CATARACTS