Work

Highly Sensitive Assay for Human Herpesvirus 8 Antibodies That Uses a Multiple Antigenic Peptide Derived from Open Reading Frame K8.1

Year: 2002

Type: article

Abstract: The immunodominant region of the human herpesvirus 8 (HHV-8), the antibody-binding site of glycoprotein K8.1A, was mapped to the N-terminal region by using overlapping peptides and a residue replacement method. The main epitope was located within residues 44 to 56 (GQVYQDWL----C). Based on this information, we developed an enzyme immunoassay to detect HHV-8 antibodies in human sera using a four-branch multiple antigenic peptide as the antigen. The sensitivity and specificity of the assay were 96 and 99.4%, respectively. This assay should be useful for population-based, epidemiological studies of HHV-8 infection.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.40.02.325-329.2002

Authors: Lee L. Lam, Chou-Pong Pau, Sheila C. Dollard, Philip E. Pellett, Thomas J. Spira

Institutions: Centers for Disease Control and Prevention, National Center for Infectious Diseases

Language: English

Topic: Viral-associated cancers and disorders

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W1964315214

FWCI: 1.626

Cites: 31

Cited by: 34

Related to: 10