Year: 2013
Type: article
Abstract: Radioimmunotherapy (RIT) takes advantage of the specificity and affinity of the antigen-antibody interaction to deliver microbicidal radioactive nuclides to a site of infection. In this study, we investigated the microbicidal properties of an alpha particle-emitting 213Bi-labeled monoclonal antibody (MAb), EA2-1 (213Bi-EA2-1), that binds to the immunodominant antigen on Bacillus anthracis spores. Our results showed that dormant spores were resistant to 213Bi-EA2-1. Significant spore killing was observed following treatment with EA2-1 labeled with 300 μCi 213Bi; however, this effect was not dependent on the MAb. In contrast, when spores were germinating, 213Bi-EA2-1 mediated MAb-specific killing in a dose-dependent manner. Dormant spores are very resistant to RIT, and RIT should focus on targeting vegetative cells and germinating spores.
DOI: 10.1128/aac.01266-13
Authors: Johanna Rivera, Alfred Morgenstern, Frank Bruchertseifer, John F. Kearney, Charles L. Turnbough, Ekaterina Dadachova, Arturo Casadevall
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Awards: G136037969, G1948673602, G2225302221, G2436179761, Synergy of Host Defense Mechanisms in the Lung, G2773611754, G2861005390, G4559384, G4617214234, G5343461384, G5683267944, Protective antibodies for Cryptococcal Infections, G6928497940, Biology of Fungal Melanin, G7479519539, G813456599, G8632159536, Northeast Biodefense Center, G8814259252, Molecular Characterization of C. neoformans Antibodies
OpenAlex ID: W1964392044